• B-Tg(hCD300E) mice

      C57BL/6N-Tg(RP11-354A1)1/Bcgen • 113070

      B-Tg(hCD300E) mice

      Product nameB-Tg(hCD300E) mice
      Catalog number113070
      Strain nameC57BL/6N-Tg(RP11-354A1)1/Bcgen
      Strain backgroundC57BL/6N
      NCBI gene ID (Human)
      AliasesCLM2; CLM-2; IREM2; PIgR2; IREM-2; PIgR-2; CD300LE; CMRF35-A5

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      • Targeting strategy
      • Phenotypic analysis

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        发表文章

          Targeting strategy

          Gene targeting strategy for B-Tg(hCD300E) mice. The full coding sequences of human CD300E gene that is driven by human CD300E promoter are inserted mouse genomic by BAC transgene(RP11-354A1 via SwaI ).

          Protein expression analysis in monocytes

          CD300E expression analysis in F1 B-Tg(hCD300E) mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and F1 B-Tg(hCD300E) mice, and analyzed by flow cytometry with anti-CD300E antibody (BD Biosesciences, 566315). hCD300E was exclusively detectable in monocytes in 1TG0020-0034 TG mice line.

          Protein expression analysis in macrophages

          CD300E expression analysis in F1 B-Tg(hCD300E) mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and F1 B-Tg(hCD300E) mice, and analyzed by flow cytometry with anti-CD300E antibody (BD Biosesciences, 566315). hCD300E was exclusively detectable in macrophages in 1TG0020-0034 TG mice line.

          Protein expression analysis in neutrophils

          CD300E expression analysis in F1 B-Tg(hCD300E) mice by flow cytometry. Blood cells were collected from wild-type C57BL/6 mice (+/+) and F1 B-Tg(hCD300E) mice, and analyzed by flow cytometry with anti-CD300E antibody (BD Biosesciences, 566315). hCD300E was not detectable in neutrophils.

          * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Tg(hCD300E) mice] (Cat# 113070) was purchased from Biocytogen.
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