C57BL/6-Ctla4tm1(CTLA4)Bcgen Il2ratm1(IL2RA)Bcgen/Bcgen • 111851
| Product name | B-hCTLA4/hIL2RA mice |
|---|---|
| Catalog number | 111851 |
| Strain name | C57BL/6-Ctla4tm1(CTLA4)Bcgen Il2ratm1(IL2RA)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | (Human) |
| Aliases | CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3; p55; CD25; IL2R; IMD41; TCGFR; IDDM10 |
Gene targeting strategy for B-hCTLA4/hIL2RA mice. The exons 2-6 of mouse Il2ra gene that encode the extracellular domain were replaced by human IL2RA exons 2-6 in B-hCTLA4/hIL2RA mice. The exon 2 of mouse Ctla4 gene that encode the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hIL2RA mice.
Strain specific CTLA4 and IL2RA expression analysis in homozygous B-hCTLA4/hIL2RA mice by FACS. Splenocytes were collected from wild type (+/+) and homozygous B-hCTLA4/hIL2RA mice (H/H,H/H) stimulated with anti-mCD3ε antibody in vivo, and analyzed by FACS with species-specific anti-CTLA4 or anti-IL2RA antibodies. Mouse CTLA4 and IL2RA were detectable in wild type mice. Human CTLA4 and IL2RA were exclusively detectable in homozygous B-hCTLA4/hIL2RA mice but not in wild type mice.
